FIG 13.

GRK inhibitors decrease TJ border staining and cell number and increase cell size. (A) IF maximum projected images of total occludin and ZO-1 from DMSO control-, GRK Inhib 22-, and ABMH-treated parental MDCK cells (bar = 50 μm). (B and C) Semiquantitative scoring (see Materials and Methods) of occludin and ZO-1 border continuity following GRK Inhib 22 (B) or ABMH (C) treatment. (D and E) Nucleus counts (D) and quantification of the area within the AJ (E) of DMSO control- versus GRK Inhib 22- or ABMH-treated parental MDCK cells. Drug was present from the beginning of each run and replenished every 24 h along with fresh medium (averages from 14 [B and C] or 4 [D and E] images per condition). *, P < 0.05 compared to WT Occ or the DMSO control.