FIG 2.

Deletion of BSCL2 enhances UCP1 expression during brown adipogenesis in vitro. (A and B) Analysis of brown adipocyte marker gene expression by qPCR (A) and Western blotting (B) during brown adipogenesis in immortalized Bscl2^+/+ (+/+) and Bscl2^−/− (−/−) preadipocytes. Data are representative of three independent experiments performed in triplicate. *, P < 0.05; **, P < 0.005 versus D0. (C) Representative images of mitochondrial staining by MitoTracker Red in D7 Bscl2^+/+ and Bscl2^−/− brown adipocytes.