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. 2016 Jul 14;36(15):2078–2088. doi: 10.1128/MCB.01062-15

FIG 3.

FIG 3

Calpain knockdown enhances sensitivity to 17AAG in vitro and in vivo. (A and B) MDA-MB-231 cells transduced with a control lentivirus or a lentivirus expressing shRNA-Capns1 were subjected to transwell migration (A) or Matrigel invasion (B) assays for 8 or 24 h in the presence of the vehicle or the concentrations of 17AAG indicated, respectively. Bar graphs show the average number of cells in five random fields ± the SD. (C) MDA-MB-231 cells transduced with a control or shRNA-Capns1-expressing lentivirus were injected into the mammary glands of Rag2γ−/−/IL2Rγc−/− mice and treated with the vehicle or 17AAG. Curves represent mean tumor volumes ± the standard error of the mean for five mice in each of the cohorts indicated. Single-factor ANOVA of the k value (growth rate; P = 7.4 × 10−4) or doubling time (P = 6.36 × 10−4) indicated significant differences between all of the cohorts except the 17AAG and calpain knockdown cohorts. (D) Average numbers of lung metastases in each mouse ± SD are indicated. *, significantly different cohorts (P ≤ 0.01).