Skip to main content
. 2015 Jul 28;1(6):e23. doi: 10.1097/TXD.0000000000000533

FIGURE 2.

FIGURE 2

Analysis of ZFN-mediated GGTA1-KO. A, Flow cytometric analysis of fibroblasts from WT controls and both clones (K746 and K747) after incubation with FITC-conjugated isolectin B4 or without (WT negative control). B, Sequencing analysis of the ZFN target region within the GGTA1 gene. The original wild-type sequence is given in the upper line. Both cloned pigs (K746 and K747) carry identical mononucleotide deletion on one and dinucleotide deletion on the other allele (boxed region). Bold letters represent ZFN target sequences.