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Protection of triple transgenic fibroblasts from antibody/complement-mediated lysis. The 51Cr-labeled fibroblasts from 2 GGTA1-KO/hHO-1/hA20 pigs (K746; K747) and 2 wild-type controls (WTa; WTb) were incubated with increasing concentrations of human antibody/complement. The amount of radioactivity released into the supernatant was determined after 4 hours by measuring an aliquot of 25 μL. The percentage of specific lysis was calculated as described in Material and Methods.
