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. 2016 Mar 31;100(1):213–222. doi: 10.1189/jlb.4A1015-483R

Figure 3. Neutrophil regulation of hyphal growth and ROS production in vitro.

Figure 3.

(A and B) Growth of dsRed expressing A. fumigatus after 18 h incubation with bone marrow neutrophils from C57BL/6 mice (A) or with human peripheral blood neutrophils (B) in the presence of Stattic, Ruxolitinib, or SR1001. Fungal mass was measured by fluorimetry of dsRed and represented as RFU. Controls are medium only [no neutrophils (no neuts)] and neutrophils that were not stimulated with IL-6/23. (C and D) ROS production (intracellular CFDA) by IL-6/23-stimulated murine and human neutrophils. MFI, Mean fluorescence intensity. Controls are unstimulated neutrophils (no hyphae). All wells are the means ± sd of 3 wells/experimental condition from neutrophils pooled from 3 mice/group (A and C) or from a single donor (B and D). Data are representative of 2 separate experiments. *P < 0.01.