Table 2.
Proportions of neurons immunoreactive for neuropeptides or GRP-EGFP.
| Laminae I–II |
Lamina III |
|||||||
|---|---|---|---|---|---|---|---|---|
| Total number of neurons counted | Number of IR neurons | % of neurons that were IR | Estimated % of excitatory neurons that were IR | Total number of neurons counted | Number of IR neurons | % of neurons that were IR | Estimated % of excitatory neurons that were IR | |
| SST | 294 (259–333) | 129 (112–152) | 43.8 (42.4–45.6) | 59 | 146.3 (144–150) | 23.7 (20–27) | 16.2 (13.8–18) | |
| Neurotensin | 304.3 (294–310) | 20.3 (19–22) | 6.7 (6.1–7.1) | 9 | 144.3 (128–161) | 11.3 (8–14) | 7.8 (6.3–9.7) | 12.5 |
| PPTB | 350 (335–374) | 40.7 (33–47) | 11.6 (9.9–12.6) | 15.6 | 152 (134–174) | 6.3 (4–8) | 4.1 (3–4.7) | 6.6 |
| GRP–EGFP | 616 (613–618) | 68 (63–72) | 11 (10.2–11.8) | 14.8 | ||||
The table shows the total number of neurons counted, together with the number and percentage that were immunoreactive (IR) for each peptide. GRP-EGFP cells were rarely seen in lamina III and so were not counted in this lamina. In each case, the mean values for three mice are shown, with the range in brackets. The estimates of percentage of excitatory neurons in each lamina that were accounted for by each neurochemical population are based on the data from literature.73 Because an unknown proportion of SST cells in lamina III are inhibitory (GABAergic/glycinergic25,32), the proportion has not been estimated for SST cells in lamina III.