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. 2016 Jun 13;107(7):972–980. doi: 10.1111/cas.12962

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© 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Distribution of Pragmin and C‐terminal Src kinase (Csk) to focal adhesions. (a,b) Confocal x‐y images are shown. Scale bar = 50 μm. (a) Immunostaining analysis of AGS human gastric epithelial cells infected with indicated lentiviruses. Anti‐Myc antibody (green), anti‐Flag antibody (red), DAPI (blue). (b) Immunostaining analysis of AGS cells. Upper, anti‐Pragmin antibody (green), anti‐vinculin antibody (red), DAPI (blue). Lower, anti‐Csk antibody (green), anti‐vinculin antibody (red), DAPI (blue). (c) Immunoprecipitation (IP)–immunoblot analysis of endogenous Pragmin in AGS cells. Arrowhead indicates the position of Csk. TCL, total cell lysates.