Figure 7.

(a) Effect of panobinostat and/or ponatinib on the acetylation status of histone H3 and on the expression level of heat shock protein (HSP)70 in CML cell lines. All cell lines were treated for 24 h with panobinostat, ponatinib, or their combination at the indicated concentrations. Western blotting was then carried out to evaluate the protein expression levels of histone H3 and acetylated histone H3. β‐Actin was assayed as a loading control. (b) Western blotting was also used to evaluate the protein expression levels of HSP90 and HSP70 in K562 cells at 24 and 18 h, respectively, after treatment with 10 nM panobinostat, ponatinib, or their combination.