Figure 2.

Ileal NLRP6 location is altered in inflammatory bowel disease [IBD]. [A] NLRP6 gene expression in ileal biopsies as measured by quantitative reverse-transcription polymerase chain reaction [qRT-PCR] [relative quantification, RQ]. Data are expressed as median with interquartile range, minimum and maximum. [B] Representative immunofluorescence of biopsies stained for NLRP6 protein showed expression in the lamina propria and proportionately distributed throughout epithelial cells of non-IBD group, with lower and intermittent epithelial distribution in both IBD subsets. Lu, lumen; LP, lamina propria; arrowhead, NLRP6-expressing cell in the LP; bar is 50 µm. The dashed lines were digitally added to mark the epithelial surface.