. 2016 Jul 15;12(7):e1006133. doi: 10.1371/journal.pgen.1006133

Table 1. Targeted screen for factors regulating Ptbb-6::GFP expression and larval development in isp-1(qm150) worms (quantified relative to vector-treated animals).

RNAi	Gene Name	Gene Function	Ptbb-6::GFP	Development	Reference
*MAPKs*
B0478.1	jnk-1	Jun-N-terminal MAPK (stress response)	$+$	no effect
T07A9.3	kgb-1	Jun-N-terminal MAPK (stress response)	no effect	no effect
ZC416.4	kgb-2	Jun-N-terminal MAPK (stress response)	no effect	no effect
C49C3.10		Jun-N-terminal MAPK (stress response)	no effect	no effect
Y51B9A.9		Jun-N-terminal MAPK (stress response)	no effect	no effect
B0218.3	pmk-1	p38 MAPK (stress response)	no effect	no effect
F42G8.3	pmk-2	p38 MAPK (stress response)	no effect	no effect
F42G8.4	pmk-3	p38 MAPK (stress response)	$---$	no effect
F43C1.2	mpk-1	ERK MAPK (growth response factor)	no effect	no effect
W06B3.2	sma-5	ERK MAPK (development)	$+++$	delay/arrest
W06F12.1	lit-1	nmo MAPK (development and inflammation)	no effect	no effect
C04G6.1	mpk-2	MAPK	no effect	no effect
C05D10.2		MAPK	$-$	no effect
F09C12.2		related to MAPK	$-$	no effect
*MAP2Ks*
F35C8.3	jkk-1	MAPKK	$-$	no effect
K08A8.1	mek-1	MAPKK	no effect	no effect
Y54E10BL.6	mek-2	MAPKK	variable	no effect
F42G10.2	mkk-4	MAPKK	$+$	no effect
R03G5.2	sek-1	MAPKK	$--$	no effect
ZC449.3	sek-3	MAPKK	$---$	no effect
F35C8.2	sek-4	MAPKK	no effect	no effect
F35C8.1	sek-5	MAPKK	no effect	no effect
VZC374L.1	sek-6	MAPKK	no effect	no effect
E02D9.1		MAPKK	no effect	no effect
*MAP3Ks*
F29C4.1	daf-1	MAPKKK	no effect	no effect
C05D2.1	daf-4	MAPKKK	no effect	no effect
F33E2.2	dlk-1	MAPKKK	$--$	no effect
F13B9.5	ksr-1	MAPKKK	no effect	no effect
F58D5.4	ksr-2	MAPKKK	no effect	no effect
K11D12.10	mlk-1	MAPKKK	no effect	no effect
F52F12.3	mom-4	MAPKKK	no effect	no effect
B0414.7	mtk-1	MAPKKK	no effect	no effect
F59A6.1	nsy-1	MAPKKK	no effect	no effect
K09B11.1	pik-1	MAPKKK	no effect	no effect
C24A1.3		MAPKKK	no effect	no effect
Y105C5A.x		MAPKKK	no effect	no effect
*Dual-Specificity Phosphatases*
F08B1.1	vhp-1	dual-specificity MAPK phosphatase	$+++$	arrested	[61]
C04F12.8		potential dual-specificity MAPK phosphatase	no effect	no effect
C24F3.2		potential dual-specificity MAPK phosphatase	no effect	no effect
F13D11.3		potential dual-specificity MAPK phosphatase	no effect	no effect
F28C6.8		potential dual-specificity MAPK phosphatase	no effect	no effect
Y54F10BM.13		potential dual-specificity MAPK phosphatase	$+$	delayed
ZK757.2		potential dual-specificity MAPK phosphatase	no effect	no effect
*PMK-3 Signaling Pathway Interactors*
D1005.3	cebp-1	bZIP TF; CCAAT-enhancer binding protein	no effect	no effect	[62]
C44C8.6	mak-2	MAP kinase activated protein kinase	$+$	delayed	[63]
F26H9.6^*	rab-5	RAB5 GTPase ortholog	$+$	arrested	[64]
C01B7.6^*	rpm-1	E3 ubiquitin ligase	$-$	no effect	[65]
F26H9.7	uev-3	ubiquitin-conjugating enzyme (E2) variant	$--$	no effect	[63]
*Transcription Factors modulating Mit Mutant Lifespan*
C25A1.11	aha-1	AHA-1 interacts with AHR-1 and HIF-1 in vitro	no effect	delayed	[33]
ZC64.3	ceh-18	POU-class homeodomain transcription factor	$+$	asynchronous	[33]
ZK652.5^*	ceh-23	homeodomain transcription factor	no effect	no effect	[66]
F52B5.5	cep-1	ortholog of human tumor suppressor p53	no effect	no effect	[21]
F38A6.3	hif-1	hypoxia-induced transcription factor	no effect	no effect	[67]
W02C12.3^*	hlh-30	bHLH TF; lipid metabolism	no effect	delayed	[68]
T24H10.7	jun-1	bZIP TF; development	$+$	no effect	[33]
F16H9.2	nhr-27	nuclear hormone receptor transcription factor	no effect	no effect	[33]
K10C3.6	nhr-49	NHR transcription factor; lipid metabolism	no effect	asynchronous	[33]
R119.6	taf-4	TFIID transcription factor	$+++$	delayed	[33]
*Immune Response*
C33D3.1^*	elt-2	GATA-type TF; intestinal immunity	no effect	delay/arrest	[69]
C50H2.1	fshr-1	neuropeptide receptor	no effect	no effect	[70]
Y53C10A.12	hsf-1	heat-shock TF; stress and immune response	$++$	$not tested$	[71]
K02F3.4	zip-2	immune response	no effect	no effect	[72]
*spg-7 RNAi Induced*
F45E4.1^*	arf-1.1	ADP-ribosylation factor	no effect	no effect	[43]
ZC376.7	atfs-1	mitochondrial unfolded protein	$+++$	delay/arrest	[43]
K01D12.11^*	cdr-4	cadmium responsive	$+$	no effect	[43]
F52E1.13^*	lmd-3	oxidative resistance	$+$	no effect	[43]
F40F8.7	pqm-1	paraquat responsive	$+$	no effect	[43]
T19E7.2	skn-1	development; oxidative stress response	$+$	no effect	[43]
F47H4.10^*	skr-5	homolog of Skp1 in S. cerevisiae	no effect	no effect	[43]
T16G1.4^*		uncharacterized	$+$	no effect	[43]
*Cytoprotection*
F57H12.1	arf-3	ADP-ribosylation factor	$+++$	no effect	[60]
F56A8.6^*	cpf-2	mRNA cleavage	$+++$	delayed	[60]
F09G2.4^*	cpsf-2	cleavage and polyadenylation specificity factor	$+$	delayed	[60]
D2045.6	cul-1	cullin; development	$+++$	delayed	[60]
R13H8.1	daf-16	forkhead box O (FOXO) transcription factor	no effect	no effect	[60]
C26C6.5^*	dcp-66	ortholog of NuRD component p66	no effect	no effect	[60]
F47A4.2	dpy-22	mediator protein subunit	$-$	no effect	[60]
C33D3.1^*	elt-2	GATA-type TF; intestinal immunity	no effect	delay/arrest	[60]
H13N06.3^*	gob-1	trehalose-6-phosphatase	variable	delayed	[60]
C53A5.3^*	hda-1	histone deacetylase	$-$	no effect	[60]
F25B4.6	hmgs-1	HMG-CoA synthase	$+++$	delay/arrest	[60]
F32E10.4^*	ima-3	importin alpha nuclear transport factor	$++$	delayed	[60]
C41C4.4^*	ire-1	ER unfolded protein response (UPR)	$+$	delayed	[60]
M7.1	let-70	E2 ubiquitin conjugating enzyme	$---$	delay/arrest	[60]
F38H4.9^*	let-92	catalytic subunit of protein phosphatase 2A	$---$	delay/arrest	[60]
T27C4.4^*	lin-40	component of NuRD complex	$--$	delayed	[60]
C25H3.6^*	mdt-26	mediator; development	$+$	no effect	[60]
ZC581.1^*	nekl-2	serine threonine protein kinase	$-$	arrested	[60]
T23H2.5^*	rab-10	RAB-like GTPase	$+++$	no effect	[60]
C35C5.1^*	sdc-2	regulates X transcription	no effect	delayed	[60]
F46A9.5^*	skr-1	ubiquitin ligase complex component	no effect	no effect	[60]
C06A8.2^*	snpc-1.1	small nuclear RNA activating complex	$--$	delayed	[60]
C23H3.4	sptl-1	serine palmitoyltransferase; development	$+$	arrested	[60]
F19B6.2	ufd-1	ubiquitin selection chaperone	$--$	delay/arrest	[60]
C46C2.1^*	wnk-1	WNK-type protein kinase homolog	no effect	no effect	[60]
F53F4.11^*		an ortholog of human RSL1D1	no effect	delayed	[60]
*Surveillance*
F40F12.7	cbp-3	CREB binding protein	$---$	arrested	[26]
F31E3.1	ceh-20	homeodomain transcription factor	$-$	no effect	[26]
Y47G6A.23	lpd-3	lipid metabolism	variable	no effect	[26]
R05D11.3	ran-4	nuclear transport factor; development	variable	delayed	[26]
Y54E10BR.5		signal peptidase complex subunit	no effect	no effect	[26]
Mitochondria-associated degradation (MAD)
C06A1.1	cdc-48.1	AAA-ATPase	$-$	arrested
C41C4.8	cdc-48.2	AAA-ATPase	$-$	arrested
F59E12.5	npl-4.2	ubiquitin selection chaperone	$--$	arrested
F19B6.2	ufd-1	ubiquitin selection chaperone	$--$	delay/arrest	[60]
K06H7.3	vms-1	VCP/Cdc48-associated (controversial role)	no effect	no effect	[73–75]
*Other*
Y116A8C.12^*	arf-6	ADP-ribosylation factor	$-$	no effect
C06A1.1	cdc-48.1	ubiquitin selection chaperone; ERAD	$-$	arrested
C41C4.8	cdc-48.2	ubiquitin selection chaperone; ERAD	$-$	arrested
C35D10.9	ced-4	programmed cell death	no effect	no effect	[76]
F56D2.7	ced-6	cell-corpse engulfment during apoptosis	no effect	no effect
R13H8.1	daf-16	forkhead box O (FOXO) transcription factor	no effect	no effect
C26D10.5	eff-1	involved in cell fusions	no effect	no effect
F52E1.7	hsp-17	heat-shock protein chaperone	no effect	no effect
C09H6.2^*	lin-10	required for polarized protein localization	$+$	no effect
F59E12.5	npl-4.2	ER-associated protein degradation (ERAD)	$-$	arrested
F55B12.5	nrf-5	lipid-binding transportation protein	no effect	no effect
F29B9.4	psr-1	apoptotic pathway	no effect	no effect
C03C10.4	rei-1	RAB-11 GEF activity	$+++$	no effect	[77]
F10D11.1	sod-2	mitochondrial superoxide dismutase	$-$	no effect	[78]
C44H4.5^*	tap-1	TGF-beta activated kinase	no effect	no effect
F42D1.2	tatn-1	tyrosine amino transferase	no effect	no effect
T04H1.9	tbb-6	beta-tubulin	no effect	no effect	[43]
R13F6.4	ten-1	teneurin	$--$	no effect
ZK524.2	unc-13	regulator of neurotransmitter release	$+$	no effect	[79]
K06H7.3	vms-1	VCP/Cdc48-associated mito stress responsive	no effect	no effect	[73]
D2030.9	wdr-23	negative regulator of SKN-1	$-$	delayed	[80]
T20F10.1	wts-1	integrity of apical intestinal membrane	no effect	no effect
C03C10.4		mitochondrial ribosome interacting protein	$+++$	no effect

* RNAi clone was sequence not verified