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. 2016 Jul 15;11(7):e0158977. doi: 10.1371/journal.pone.0158977

Fig 6.

Fig 6

Immunohistochemical detection of uromodulin (UMOD, a-d), POU3F3 (e-l), and Aquaporin 2 (AQP2, m-r) in kidney sections of 60-week-old homozygous Pou3f3L423P mutant mice (HOM) and non-mutant control mice (CON). Positive immunoreactivity (UMOD, POU3F3, AQP2 in a-h and m-r) is indicated by brown color (chromogen: DAB). In the double-immunohistochemical detection (i-l), UMOD immunoreactivity is indicated by blue color (chromogen: NBT/BCIP) and POU3F3 by brown color (DAB). Arrows mark the TAL and the MD, CDs are indicated by hashs, and asterisks mark glomeruli. Black rectangles indicate the positions of the detail enlargements. Positive UMOD immunoreactivity with a diffuse cytoplasmic and distinct apical membrane staining pattern is present in epithelial cells of the TAL, POU3F3 immunoreactivity is present in the TAL, the MD and the CDs (nuclear staining pattern), and CD cells display a cytoplasmic staining pattern for the CD marker AQP2. Paraffin sections. Nuclear counterstain: Hemalum. Bars = 1 mm (left row), = 50 μm (middle and right row).