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. 2016 May 11;94(6):139. doi: 10.1095/biolreprod.115.137554

FIG. 2.

FIG. 2

Changes in eNOS phosphorylation in response to ERα activation in UAECs. A) At 20 min, PPT (ERα agonist) increased eNOS stimulatory phosphorylation of Ser1177eNOS to similar levels as E2β. B) E2β increased stimulatory phosphorylation of Ser635eNOS whereas only the highest PPT concentration induced this increase. C) E2β and PPT decreased eNOS inhibitory phosphorylation at site Thr495eNOS. D) Representative Western blots for phosphorylated eNOS, total eNOS, and β-actin. Cnt, control. *P < 0.05.