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. 2016 May 11;94(6):139. doi: 10.1095/biolreprod.115.137554

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© 2016 by the Society for the Study of Reproduction, Inc.

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FIG. 3 — Changes in eNOS phosphorylation in response to ERβ activation in UAECs. A) At 20 min, E₂β increased ^Ser1177eNOS stimulatory phosphorylation by 1.35-fold whereas DPN had no effect on this site. B) E₂β increased ^Ser635eNOS stimulatory phosphorylation by 1.48-fold whereas DPN did not increase phosphorylation at this site. C) E₂β induced a decrease in the inhibitory phosphorylation at site ^Thr495eNOS by 0.84-fold. DPN also decreased the inhibitory phosphorylation of ^Thr495eNOS to 0.82-fold at 0.1 nmol/L, 0.82-fold at 1.0 nmol/L, 0.84-fold at 10 nmol/L, and 0.85-fold at 50 nmol/L. D) Representative Western blots for phosphorylated eNOS, total eNOS, and β-actin. Cnt, control. *P < 0.05.