Table 1.

Candidate genes enriched in the eight independent screens for genes involved in centrosome loss-induced G1 arrest.

DOI: http://dx.doi.org/10.7554/eLife.16270.008

Enrichment compared to p53	Gene symbol	Gene name or description	Number of sgRNAs enriched out of total 6
1.0000	TP53	Tumor Protein P53	5/6
0.9386	TP53BP1	Tumor Protein P53 Binding Protein 1	4/6
0.9039	TRIM37	Tripartite Motif Containing 37	3/6
0.8895	USP28	Ubiquitin Specific Peptidase 28	4/6
0.8858	P21	Cyclin-Dependent Kinase Inhibitor 1A (P21, Cip1)	6/6
0.7559	CHD8	Chromodomain Helicase DNA Binding Protein 8	4/6
0.6945	FBXO42	F-Box Protein 42	3/6
0.6846	RNF20	Ring Finger Protein 20, E3 Ubiquitin Protein Ligase	3/6
0.6665	PCDHGC5	Protocadherin Gamma Subfamily C, 5	2/6
0.6604	TSPY8	Testis Specific Protein, Y-Linked 8	3/6
0.6395	RWDD3	RWD Domain Containing 3	2/6
0.6364	UGT1A9	UDP Glucuronosyltransferase 1 Family, Polypeptide A9	2/6
0.6285	PDSS1	Prenyl (Decaprenyl) Diphosphate Synthase, Subunit 1	2/6
0.6275	BPIFB4	BPI Fold Containing Family B, Member 4	2/6
0.6240	PCDHAC2	Protocadherin Alpha Subfamily C, 2	3/6
0.6218	CSNK2A1	Casein Kinase 2, Alpha 1 Polypeptide	2/6
0.6201	GAGE7	G Antigen 7	3/6
0.6119	WTAP	Wilms Tumor 1 Associated Protein	2/6
0.6093	PRDM11	PR Domain Containing 11	2/6
0.6086	OR56A1	Olfactory Receptor, Family 56, Subfamily A, Member 1	2/6
0.6059	TBCEL	Tubulin Folding Cofactor E-Like	2/6
0.5997	DNAJC9	DnaJ (Hsp40) Homolog, Subfamily C, Member 9	2/6
0.5991	NPIPL3	Nuclear Pore Complex Interacting Protein Family, Member B3	3/6
0.5987	SYDE1	Synapse Defective 1, Rho GTPase, Homolog 1	2/6
0.5959	TECPR2	Tectonin Beta-Propeller Repeat Containing 2	2/6
0.5683	STOML3	Stomatin (EPB72)-Like 3	2/6
0.5507	DVL3	Dishevelled Segment Polarity Protein 3	2/6

Log₂ scaling of sgRNAs enrichment normalized to the top scoring hit p53. The sgRNAs for genes highlighted in red were validated in this study. For a gene to be scored as a candidate, at least two of its six sgRNAs were repeatedly enriched in independent screens, with the HiSeq reads at least 3 times higher than the average of the baseline read.