Figure 5. Summary of structure-based alanine scanning mutagenesis.
(A–D) Phenotypes of HIV-1 Gag △MA-CA-SP1-NC assembled with DNA. The ribbon diagram is of two opposing subunits in the hexamer with Cα atoms shown as spheres and color-coded according to assembly phenotypes as shown in panels B, C, and D. (E–H) Phenotypes of △MA-CA-SP1-NC assembled with tartrate. Scale bars = 150 nm.
Figure 5—figure supplement 1. Alanine scanning mutagenesis of CA-SP1.
Shown are representative images of △MA-CA-SP1-NC proteins assembled in vitro through dialysis with a single-stranded DNA template. Boxes are colored according to assembly phenotypes as indicated in Figure 5A–D and Table 2. Insets show a representative immature particle for each particular mutant, if observed. Scale bars = 150 nm.
Figure 5—figure supplement 2. Alanine scanning mutagenesis of CA-SP1.
Shown are representative images of △MA-CA-SP1-NC proteins assembled in vitro by incubating with tartrate. Boxes are colored according to assembly phenotypes as indicated in Figure 5E–H and Table 2. Scale bars = 150 nm.