. 2016 Jun 11;5:e14770. doi: 10.7554/eLife.14770

Table 2.

N and B parameters for SimFCS software analysis. SEM is given.

DOI: http://dx.doi.org/10.7554/eLife.14770.018

Table 2—source data 1. Monomeric brightness of 35S:GFP line obtained using N&B with the Zeiss 780 and Zeiss 710 instruments.

DOI: http://dx.doi.org/10.7554/eLife.14770.019

elife-14770-table2-data1.xlsx^{(13KB, xlsx)}

DOI: 10.7554/eLife.14770.019

Table 2—source data 2. S-factor of the 35S:GFP background line obtained using N&B with the Zeiss 780 and Zeiss 710 instruments.

DOI: http://dx.doi.org/10.7554/eLife.14770.020

elife-14770-table2-data2.xlsx^{(13.8KB, xlsx)}

DOI: 10.7554/eLife.14770.020

Table 2—source data 3. S-factor of the UBQ10:mCherry background line and monomeric brightness of UBQ10:mCherry line obtained using N&B with the Zeiss 780 instrument.

DOI: http://dx.doi.org/10.7554/eLife.14770.021

elife-14770-table2-data3.xlsx^{(13.8KB, xlsx)}

DOI: 10.7554/eLife.14770.021

Confocal model and objective	S-factor (green channel)	S-factor (red channel)	Monomer brightness (green channel) (counts/pixel dwell/molecule)	Monomer brightness (red channel) (counts/pixel dwell/molecule)	Cursor size
LSM 780, 40 x 1.2 NA water	1.34 ± 0.02 (n = 17)	1.00 ± 0.01 (n = 24)	0.28 ± 0.01 (n = 13)	0.34 ± 0.02 (n = 7)	42 ± 0.69 (n = 13)
LSM 710, 40 x 1.2 NA and 63 x 1.2 NA water	0.92 ± 0.004 (n = 20)	N/A^*	0.24 ± 0.01 (n = 20)	N/A^*	50 (n = 20)
Source data is provided in Figure 2—source data 1 (Monomer brightness for green channel and cursor size); Table 2—source data 2 (S-factor, green channel); and Table 2—source data 3 (Monomer brightness and S-factor, red channel) *Red channel data was not collected on the LSM 710