Target locus	Location	Primer probe	Sequence
p16	Chromosome 9: 21967752..21994491, complement	Forward primer Reverse primer Probe	5′-AACATGGTGCGCAGGTTCTT-3′ 5′-TGAACCACGAAAACCCTCACT-3′ 5′-6-FAM-CCCTCCGGATTCG-MGB-3′
p14	Chromosome 9: 21967752..21994491, complement	Forward primer Reverse primer Probe	5′-GCGGTCCCTCCAGAGGAT-3′ 5′-CGGTGCTGGCGGAAGA-3′ 5′-6-FAM-TGAGGGACAGGGTCG-MGB-3′
NF2	Chromosome 22: 29999545..30094589	Forward primer Reverse primer Probe	5′- GCCAGGCCCTGCTAGATAGC-3′ 5′-AACCTGTCCCCAAAATTACAAGAC-3′ 5′-6-FAM- CCCCGTGGCATTAC-MGB-3′
AldoB (aldolase B, reference)	Chromosome 9: 101420560..101435780, complement	Forward primer Reverse primer Probe	5′-TTTCCACGAGACCCTCTACCA-3′ 5′-CTTTTCCTTGAGGATGTTTCTGAAC-3′ 5′-6-FAM- AAGGACAGCCAGGGAA-MGB-3′
Svil (supervillin, reference)	Chromosome 10: 29457338..29736935	Forward primer Reverse primer Probe	5′-GCCTGCGGAGCGTCAA-3′ 5′-GGCACGGCGCTGTTGT-3′ 5′-6-FAM-ACGGAACAGAACTCT-MGB-3′