Figure 3.

Aiolos/Blimp-1 and Aiolos/Ikaros heterodimers bind to two distinct motifs. (a) Sequences shown are the Blimp-1- and Aiolos-binding consensus motifs analyzed with the motif discovery web server eTFBS. (b) Lysates from H929 cells were used to perform IP with the indicated antibodies, followed by IB. Arrow indicates a nonspecific band. (c and d) Chromatin prepared from H929 cells was subjected to a ChIP and re-ChIP assay using anti-Blimp-1 (c) or anti-Ikaros (d) in the first ChIP and anti-Aiolos or rabbit IgG in the re-ChIP. Results in (c) and (d) represent the mean±S.E.M. (n=3). **P<0.01; ***P<0.005