Figure 4.

Tip60 mRNA expression correlates inversely with defective HR-directed DNA repair. (a) GSEA of the association of Tip60 (Kat5) with the HRD gene signature of Peng et al.³⁴ was performed using default values for all parameters and a specific gene set. (b) Quantitation of RT-PCR analysis of relative Tip60 mRNA levels in one group of mammary tumors with high Tip60 expression and another group of mammary tumors with low Tip60 expression. Tumors were from MMTV-PyMT and MMTV-Neu mice (n=5/group). Data were analyzed as in Figure 1d and are representative of two trials. (c) Quantitation of RT-PCR analysis of relative mRNA levels of 8 genes associated with the HRD gene signature in mammary tumors with high or low Tip60 expression. Data are expressed as absolute values for individual tumors. Horizontal lines are mean values±S.E.M. *P<0.005, **P<0.01, and ***P<0.001