Figure 4.

ANXA1 promotes virus replication in vitro. (a and b) Scramble or ANXA1-shRNA transfected A549 lung epithelial cells were infected with 1 MOI of influenza A/PR8 for the indicated time points. Virus was quantified in supernatants using qPCR for M gene expression or NS1 expression. CT values were converted to viral particles using a standard curve for M1 or NS1 expression (mean±S.D. of duplicates with at least three independent repeats). (c) Viral titers were measured in the supernatants using plaque assays (mean±S.D. of duplicates with at least three independent repeats). (d) A549 cells transfected with empty vector (EV) or ANXA1 pcDNA3.1-V5 (ANXA1-V5) were infected with 1 MOI of influenza A/PR8. Virus was quantified in supernatants using qPCR for M1 gene expression. CT values were converted to viral particles using a standard curve for M1 expression (mean±S.E. of three independent experiments. *P<0.05; **P<0.01 versus Scr-sh or EV at the same time points