Figure 4. RNA selectivity of CA domain mutants.
(a) Confocal micrograph of GUV, 10 min after adding 100 nM Gag-ATTO594 and 0.5 nM 5’UTR -Alexa488. Upper panel, membrane in red and Gag in white/cyan. Lower panel, RNA. (b–e) As (a) using Gag two-fold, Gag three-fold, Gag six-fold and Gag two-, three-, six-fold, respectively. (f–j) As (a–j) with the addition of 5 nM non-fluorescent RNA378. (a–j) White arrows mark Gag clusters on GUVs, and their position on the corresponding RNA images. Scale bar for all images, 10 µm. (k) Quantitation of RNA binding to Gag clusters, performed as for Figure 1g. n.s./*, not significant, and significant, respectively, at p<0.05 level by Student’s t-test.
Figure 4—figure supplement 1. Type of Gag binding to GUVs in the presence of RNA.
Statistics of Gag membrane binding was performed as for Figure 3g, using the data presented in Figure 4f–j (ten z-stacks per condition, in three independent repeats).