FIGURE 4.

Dileucine in the linker region of Drb1 functions as a nuclear export signal. A, schematic of Drb1 deletion and missense mutants. B, subcellular localization of Clover (a–c) and Clover-T7 fused WT (d–f), 2–400 (g–i), 2–300 (j–l), 301–474 (m–o), 301–400 (p–r), and 301–400-mtLL (s–u) Drb1 in HeLa cells. The representative subcellular localization of the expressed protein is shown on the right as nucleoplasm (N) and cytoplasm (C), respectively. The nuclei were stained with Hoechst 33258. Scale bars = 10 μm.