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Thermal melting temperature shifts induced by fenamic acid inhibitor binding to COX-2. huCOX-2 was incubated with 50 μm concentrations of the indicated inhibitor before measuring the melting temperature as described under “Experimental Procedures.” Results are reported as the difference in melting temperature (ΔTm) between ligand bound and apo states of COX-2. Melting temperature measurements were made in triplicate ± S.E.
