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Tryptophan fluorescence quenching of huCOX-2 by fenamic acids. Fenamic acid derivatives were added after 30 s to 0.26 μm huCOX-2 to a final concentration of 1 μm, and native tryptophan fluorescence was monitored with λex 283 nm and λem 335 nm as described under “Experimental Procedures.” Measurements were carried out in triplicate.
