TABLE 2.

Calculated kinetic parameters for flufenamic acid and tolfenamic acid inhibition of huCOX-2 as a function of inhibitor concentration

Measurements were carried out in duplicate ± S.D.

Tolfenamic acid			Flufenamic acid
[Inhibitor]	Km	VMAX	[Inhibitor]	Km	VMAX
nm	μm	>nmol O2/min	nm	μm	nmol O2/min
0	14.4 ± 2.6	146.7 ± 14.1	0	12.8 ± 1.9	141.6 ± 10.7
50	12.0 ± 2.9	121.8 ± 16.6	100	10.1 ± 1.9	111.8 ± 10.3
100	7.3 ± 3.2	78.0 ± 17.5	300	6.0 ± 2.4	70.1 ± 13.5
300	2.4 ± 1.4	27.5 ± 7.1	500	3.5 ± 1.7	27.4 ± 5.2