FIGURE 2.
Inhibition of tankyrase stabilizes AMOT family proteins. A, proteomics strategy to identify tankyrase substrates. B, scatter plot depicting proteins identified and quantified in a quantitative proteomics experiment. Proteins significantly depleted in XAV939-treated condition are highlighted as potential substrates of tankyrase. C, tankyrase inhibitors increase protein levels of AMOT p130 and AMOTL1. D, depletion of TNKS1/TNKS2 or RNF146 increases the protein level of AMOT p130. E, proteasome inhibitor MG132 increases protein levels of AMOT p130 and AMOTL1. F, tankyrase inhibitor increases half-life of HA-AMOTL1. G, tankyrase inhibitor suppresses PARsylation of AMOT p130.