Skip to main content
. 2016 May 26;291(29):15256–15266. doi: 10.1074/jbc.M116.722967

FIGURE 6.

FIGURE 6.

Stabilization of angiomotins is required for growth inhibitory activity of tankyrase inhibitor in NSCLC. A–C, knock-out of YAP sensitizes cells to the growth inhibitory activity of Erlotinib. PC9 stably expressing Cas9 were infected with lentivirus expressing control gRNA or gRNA against YAP. Cells were subjected to Western blot assay (A), CTG assay (B), or colony formation assay (C) in combination with different doses of Erlotinib. For proliferation assays, error bars are S.E., n = 3. Data are representative from at least three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001; NS, not significant. D–G, knock-out of angiomotins attenuates YAP inhibitory and growth inhibitory activities of tankyrase inhibitor. PC9-Cas9 cells expressing control gRNA or gRNAs against angiomotins were treated with indicated compound, and subjected to Western blot assay (D), qRT-PCR assay (E), CTG assay (F), or colony formation assay (G).