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. 2016 May 16;291(29):15307–15319. doi: 10.1074/jbc.M116.735134

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Mot1 and Spt16 genetically and physically interact. A, 10-fold serial dilutions of the indicated strains were plated on rich medium and incubated at the indicated temperatures for 3 days. The mot1-42 spt16-197 strain exhibited synthetic sickness at 30 °C compared with the other strains. B, co-IP of Spt16 and Mot1 in whole cell extracts obtained from the strains with HA-tagged Spt16 and/or myc-tagged Mot1 as indicated. IPs were performed using the myc antibody, and blots were probed with the antibody indicated to the left of each panel. WCE lanes show results with whole cell extracts used as input in the IPs. C, a similar experiment as B using a polyclonal Spt16 antibody in the IP. IB, immunoblotting.