TABLE 1.
Kinetic parameters of the ET reaction from CcO and wild-type and mutant Cyt c
| Groupa | Km | kcat | kcat/Km | |
|---|---|---|---|---|
| μm | s−1 | μm−1s−1 | ||
| K13L | A | 12 ± 1 | 62 ± 2 | 5.3 ± 0.6 |
| K86L/K87L | A | 10 ± 1 | 71 ± 2 | 7.0 ± 0.7 |
| K7L/K8L | B | 7.0 ± 0.7 | 110 ± 10 | 16 ± 2 |
| K72L | B | 5.6 ± 1.1 | 130 ± 10 | 24 ± 5 |
| K79L | B | 5.0 ± 0.8 | 83 ± 4 | 17 ± 3 |
| I9A | C | 0.82 ± 0.15 | 46 ± 1 | 57 ± 10 |
| I11A | C | 1.1 ± 0.2 | 51 ± 1 | 45 ± 7 |
| I81A | C | 1.2 ± 0.1 | 49 ± 1 | 41 ± 2 |
| K39L | C | 1.2 ± 0.1 | 49 ± 1 | 43 ± 2 |
| Wild type | − | 1.2 ± 0.1 | 48 ± 1 | 42 ± 4 |
a Groups A–C represent severe, moderate, and unaffected mutational effects, respectively.