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. Author manuscript; available in PMC: 2016 Jul 15.
Published in final edited form as: Neurochem Int. 2012 Feb 15;60(5):506–516. doi: 10.1016/j.neuint.2012.02.007

Fig. 4.

Fig. 4

Changes in mitochondria morphology are influenced by NMDAR subunit activation. (A–F) 10 min following NMDA treatment, mitochondrion shape index decreases (A and B). The relative amount of change in mitochondria shape is maintained if the NR2A-containing NMDARs are inhibited prior to NMDA treatment (C and D). Alternatively, the shape of mitochondria is preserved by blocking NR2B containing NMDARs (E and F). (G) Significant differences in the shape factor (ΔSI), defined as the difference between pre-NMDA and post-NMDA shape measures for individual mitochondrion, were observed following NMDA treatment and were not significantly altered when NR2A-containing NMDARs were inhibited. Change in shape of mitochondria was significantly reduced in cultures when NR2B-containing NMDARs were inhibited. Blocking synaptic NMDARs with a pretreatment of bicuculline (Bic) + MK801 treatment did not prevent mitochondrial swelling in mature cultures (G) but significantly reduced swelling in younger cortical cultures (H). NVP in micrographs denote NVP-AAM077. Scale bars 10 μm.