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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Pain. 2016 Apr;157(4):806–817. doi: 10.1097/j.pain.0000000000000439

Figure 7. Quantitative RT-PCR shows the expression of Tlr4 and glia-related genes in cervical spinal cord dorsal horn (C3–C4) after AEW treatment on the back skin or intrathecal LPS injection.

Figure 7

(A) Relative expression levels of Tlr4, Gfap, and Iba1 1, 3, and 5 days after AEW treatment. *P<0.05, **P<0.01, **P<0.001, compared with corresponding vehicle control, n = 4 mice per group. (B) Relative expression levels of Gfap and Iba1 in the dorsal horn of WT and Tlr4−/− mice 5 d after AEW treatment. *P<0.05; #P<0.05, n = 5 mice per group. Note that dry skin induces Tlr4-dependent Gfap expression. (C) Relative expression of Tlr4, Gfap, and Iba1 in cervical spinal cord dorsal horn 24 h after intrathecal LPS (10 μg) injection. Data are presented as means ± S.E.M. *P<0.05, compared with corresponding vehicle control, Student’s t test; n = 4 mice per group.