. Author manuscript; available in PMC: 2016 Jul 15.

Published in final edited form as: ACS Chem Biol. 2016 May 18;11(7):1982–1991. doi: 10.1021/acschembio.6b00120

Table 2.

IC₅₀ and EC₅₀ values of second-generation AIP analogs in S. epidermidis AgrC-I determined using a fluorescence reporter strain.^a

Peptide Name	Sequence	IC₅₀ (nM)	EC₅₀^b (nM)

AIP-I A5Y	D-S-V-(C-Y-S-Y-F)	95.4	-
AIP-I A5S	D-S-V-(C-S-S-Y-F)	135^c, ^d
AIP-I V3AA5Y	D-S-A-(C-Y-S-Y-F)	59.6	-
AIP-I V3AA5S	D-S-A-(C-S-S-Y-F)	29.4	-
AIP-I D1AS6A	A-S-V-(C-A-A-Y-F)	-	10.3
tAIP-I	Ac-(C-A-S-Y-F)^e	192	-
AIP-I D1AS6AV3A	A-S-A-(C-A-A-Y-F)	2.84	-
AIP-I D1AS6AV3AA5S	A-S-A-(C-S-A-Y-F)	2.08	-
AIP-II 11aa	A-S-K-Y-N-P-(C-S-N-Y-L)	5.43	-
AIP-II 10aa	S-K-Y-N-P-(C-S-N-Y-L)	5.29	-
AIP-II 9aa	K-Y-N-P-(C-S-N-Y-L)	2.83	-
AIP-II 8aa	Y-N-P-(C-S-N-Y-L)	6.08	-
AIP-II 7aa	N-P-(C-S-N-Y-L)	721	-
tAIP-II	Ac-(C-S-N-Y-L)^e	>1000	-

^{a, b}

See footnotes in Table 1.

Maximum inhibition did not exceed 75%.

Two different AIP-I A5S analogs were isolated that exhibited differing activities; IC₅₀ value listed is derived from the antagonism dose response curves for A5S-1 (see text).

Ac = acetylated N-terminus.