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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Kidney Int. 2016 Apr 27;90(2):300–310. doi: 10.1016/j.kint.2016.02.018

Figure 1.

Figure 1

1A: Induction of hypertension in Alport mice. C57Bl/6 X-linked Alport mice were made hypertensive by giving them the L-NAME salts in their drinking water from 4 to 7 weeks of age. Normotensive mice were given plain drinking water. Blood pressures were measured longitudinally using the CODA-2 tail cuff system. Numbers on the X-axis represent individual measurements which were performed twice weekly from 4 to 7 weeks of age. Both systolic and diastolic changes comparing normotensive and hypertensive mice were stastically significant (P<0.05). 1B: Hypertension induces endothelin-1 in Alport glomerular endothelial cells. Cryosections were dual immunostained with antibodies specific for either endothelin-1 or CD31 (a marker for endothelial cells). ET-1, endothelin-1. Elevated expression of endothelin-1 is clearly evident in glomeruli from hypertensive mice relative to normotensive mice (Compare G and J). This was not observed in wild type mice (compare A and D). Co-localization of endothelin-1 with CD31 demonstrates this induction is coming from the endothelial cell compartment. Bar=30μm.