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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Dev Dyn. 2016 May 31;245(8):854–873. doi: 10.1002/dvdy.24413

Fig. 3. CELF1 is required for myofibril assembly and maintenance.

Fig. 3

(A) Western blot analysis shows that knockdown of CELF1 protein is detectable by 24 hrs post-transfection (hpt) and persists through 96 hpt. Protein integrity and equivalent loading were confirmed by Ponceau S staining (not shown) and reprobing for GAPDH. (B) Myofibrillar organization was monitored by immunofluorescence using sarcomeric markers (ACTN2, TTN, and MYOM) over 96 hrs following mock (M) or siRNA (si2) transfection. Cells were transfected at 24 hrs post-plating and fixed at 24, 48, 72, and 96 hrs post-transfection; panels match the time points indicated in (A).