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. 2016 May 20;23(7):221–228. doi: 10.1038/cgt.2016.21

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Copyright © 2016 Nature America, Inc.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Green fluorescent protein (GFP) expression in ce2 cells treated with GFP nanocomplexes. Ce2 cells were treated for 3 h with GFP DNA complexes (dosages from 5 to 20 μl) containing Listeriolysin O (LLO) and trastuzumab (Tmab; upper row); containing LLO and Tmab along with free Tmab in the medium as competitor (middle row); or containing LLO without Tmab in the complexes (lower row). Fluorescent cells were visualized 48 h after treatment with an inverted fluorescence microscope, and photographs taken with the 5 × objective. Scale bar, 500 μm.