Figure 4. Single cell sorting and gene expression profiling.

A. Gating strategy for isolation of single cells for Fluidigm Biomark single cell gene expression profiling. FSC: forward scatter. SSC: side scatter. B. Heatmap of single cell gene expression on the Fluidigm Biomark device. Columns represent single cells, and rows represent individual primer sets, with two primer sets used to detect each of the indicated genes (with the exception of Olfm4, represented by a single primer set). Total Axin2-CreERT2-tdTomato⁺ cells were sorted and analyzed, then retroactively annotated as being in the tdTomato^Low versus tdTomato^High grouping (top and bottom 50% of total tdTomato⁺ cells). A small group of Lgr5-eGFP⁺ CBCs are included in the heatmap for reference. Color intensity of the heatmap directly correlates with decrease Ct values. All data derived from the jejunum of adult mice.