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. Author manuscript; available in PMC: 2017 Jul 14.
Published in final edited form as: Cell. 2016 Jun 30;166(2):424–435. doi: 10.1016/j.cell.2016.05.071

Figure 2. Lack of classical browning and identification of increased N-oleoyl phenylalanine in mice injected with AAV-PM20D1.

Figure 2

(A and B) mRNA expression of the indicated genes in BAT, iWAT, and eWAT (A) and Western blot of UCP1 and mitochondrial proteins (B) from male C57BL/6 mice at thermoneutrality after tail vein injections of AAV-GFP or AAV-PM20D1. Mice were placed into thermoneutrality (30°C) at 6 weeks old, injected with virus at 7 weeks old, and high fat diet (HFD) was started 7 days post injection. Mice were maintained at 30°C for the duration of the experiment. For (A) and (B), data are from 47 days post injection. For (A), n=8/group, mean ± SEM, * p<0.05. For (B), n=4–5/group.

(C) Chromatogram at m/z = 428 from plasma of male C57BL/6 mice after tail vein injection of AAV-GFP or AAV-PM20D1. For (C), mice were 7 weeks old at the time of injection, high fat diet (HFD) was started 7 days post injection, and mice were maintained at room temperature for the duration of the experiment. The comparative metabolomics was performed on plasma harvested 54 days post injection. n=4/group, * p<0.05.

(D) Chemical structure of N-oleoyl phenylalanine (C18:1-Phe).

(E and F) MS/MS spectra (E) and retention time (F) of endogenous (top) or synthetic (bottom) C18:1-Phe.

See also Table S3.