FIG. 3.

3D printing sequence of the first perfusion fluidic device with an embedded 5 μm pore size cellular acetate membrane depicted in Fig. 2(b). (a) The bottom open serpentine channel was first 3D printed. (b) The cellular acetate membrane was then glued down on top of the bottom open serpentine channel. (c) 3D printing resumed after gluing down the cellular acetate membrane. (d) 3D printing completed with the cellular acetate membrane embedded between the top and the bottom open serpentine channels. Serpentine channel cross-sectional dimensions were 1 mm × 1 mm.