Fig. 8 .

Appearance of M2-type macrophages in merlin-deficient nerves after crush injury and human schwannoma samples. a Representative images of sciatic nerve cross sections from indicated genotypes. Immunolabeling of Arginase-1 as a marker for M2-type macrophages shows strong expression in P0-Cre;Nf2^fl/fl and P0-Cre;Nefh-Cre;Nf2^fl/+ mice, in comparison to wild-type (WT) littermates. Scale bars represent 100 μm. b Representative images of human schwannoma samples from a tissue microarray. Immunostainings against macrophage markers CD68 and Iba-1, as well as M2-type macrophage marker MMR/CD206, indicate macrophage occurence in sporadic, NF2-associated and Schwannomatosis-associated schwannomas. Scale bars represent 50 μm (upper panel), 10 μm (middle panel) and 20 μm (lower panel), respectively. c Cytokine levels in pooled lysates of at least four individual sciatic nerves per indicated genotype (n = 2). Densitometric quantification is shown as median value. For full-length blots see Supplementary Fig. 13