Figure 5. Opposite influence of LHL and LHF on BLP–vCA1 circuit.

(a) Protocol for measuring the influence of LHF or LHL on the activity of BLP–vCA1 circuit after vCA1 injection of retrograde monosynaptic tracers. (b,c) The representative co-staining of DsRed+/c-fos+ neurons in BLP (scale bar, 25 μm), and number of c-fos+/DsRed+ neurons in BLP after 1-day trial or 6-day trials (n=5 per group, one-way analysis of variance (ANOVA), Tukey's multiple comparisons test, *P<0.05, **P<0.01 versus Ctrl; # P<0.05, ##P<0.01 versus LHL). (d) Protocol for evaluating the structural strength of BLP–vCA1 monosynaptic connection. (e,f) The representative DsRed images in BLP neurons terminating on vCA1 (scale bar, 250 μm), and number of DsRed+ neurons in BLA (anterior part of amygdala) and BLP (posterior part of amygdala; n=5 per group, one-way ANOVA, Tukey's multiple comparisons test, *P<0.05, **P<0.01 versus Ctrl; ## P<0.01 versus LHL). (g) Infrared-differential interference contrast images of patch pipette tips on a vCA1 pyramidal neuron in a hippocampal slice (scale bar, 10 μm). (h,i) Representative EPSC_NMDA and EPSC_AMPA traces recorded from vCA1 pyramidal neuron from different groups and the quantification of EPSC_AMPA/EPSC_NMDA ratio (n=5–6 mice per group, one-way ANOVA, Tukey's multiple comparisons test, *P<0.05 versus Ctrl; ## P<0.01 versus LHL). Scale bars, 50 pA and 50 ms. (j, k) EPSP evoked in vCA1 pyramidal neuron by paired photostimuli (50-ms interpulse interval) and the quantification of PPR in slices (n=7 mice per group, one-way ANOVA, Tukey's multiple comparisons test,*P<0.05 versus Ctrl; ## P<0.01 versus LHL). Scale bars, 1 mV and 20 ms. (l,m) Representative mEPSC traces recorded from vCA1 pyramidal neurons, and the quantification of mEPSC frequency and amplitude (n=4–6 mice per group, one-way ANOVA, Tukey's multiple comparisons test, *P<0.05 versus Ctrl; ##P<0.01 versus LHL). Scale bars, 20 pA and 200 ms. Data were presented as mean±s.e.m.