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. 2016 Jul 14;7:11935. doi: 10.1038/ncomms11935

Figure 6. BLP–vCA1 activation is essential for LHF-potentiated spatial memory, and stimulating BLP–vCA1 rescues LHL-induced memory deficit and mimics the effect of LHF.

Figure 6

(a) Protocol for testing the necessity of BLP–vCA1 activation in facilitating spatial memory of LHF mice. (b–f) Simultaneous blockage of BLP–vCA1 input by NpHR light on abolishes the LHF-facilitated learning and memory on Barnes maze (BM; n=7–8 per group, two-way analysis of variance (ANOVA), Bonferroni's post hoc test, *P<0.05, **P<0.01 versus Ctrl; #P<0.05 versus light off). (h) Protocol for testing the rescue effect of BLP–vCA1 stimulation in LHL mice.(i–k) Photostimulation of BLP–vCA1 by ChR2 light on improves the LHL-induced learning and memory deficits (n=8 per group, one-way ANOVA, Tukey's multiple comparisons test, *P<0.05, **P<0.01 versus Ctrl light off; #P<0.05, ##P<0.01 versus LHL light off). (m) Protocol for testing the sufficiency of BLP–vCA1 stimulation in facilitating spatial memory in naive mice. (n–p) Photostimulation of BLP–vCA1 input by ChR2 light on for 6 days enhances spatial learning and memory (n=8 per group, unpaired t-test, *P<0.05, versus ChR2 light off). (g,l,q) Motor function was not affected (n=7–8 per group, one-way ANOVA, Tukey's multiple comparisons test or unpaired t-test). Data were presented as mean±s.e.m. Ctrl, control.