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. 2016 Jul 13;7:12124. doi: 10.1038/ncomms12124

Figure 1. Cryo-EM structure of the FANCD2–FANCI complex.

Figure 1

(a) Coomassie blue stain of recombinant full-length FANCD2–FANCI heterodimer purified from Sf9 cells. (b) Cryo-EM density map of full-length FANCD2–FANCI complex in different orientations. Scale bar, 20 Å. (c) Comparison of full-length FANCD2–FANCI Cryo-EM structure (left) with the mFANCD2–FANCI crystal structure (PDB: 3S4W) (right). The mFANCD2–FANCI crystal structure was filtered to the same resolution as the 3D Cryo-EM model. Scale bar, 20 Å. (d) Different orientations of Cryo-EM density map of full-length FANCD2–FANCI complex docked with the mFANCD2–FANCI crystal structure (PDB: 3S4W). Scale bar, 20 Å.