Figure 6. Tsc1 ablation activates cell apoptotic program.

(a,b) Representative images showing the corpus callosum of P14 control and Tsc1cKO mice immunostained with antibodies to (a) Fas and CC1, and (b) CHOP and Olig2. Scale bars, 25 μm. (c) Percentage of CHOP⁺/Olig2⁺ cells in control and Tsc1cKO cortices at P14. Data represent the mean±s.e.m. from three animals. *P<0.05; **P<0.01; ***P<0.001; Student's t-test. (d) Primary OPCs from control and Tsc1cKO pups cultured in T3-containing differentiation medium for 24 h were immunostained with CHOP and Fas. Scale bars, 25 μm. (e) Western blot analysis of extracts from primary OPCs isolated from control and Tsc1cKO animals with antibodies to Fas, CHOP, p-JNK, p-S6 and p-4EBP1; β-actin: loading control. (f) Primary OPCs from wild-type mice were treated with solvent and 100 ng ml⁻¹ FasL for 24 h, and immunostained with antibodies to cl-Casp3 and Sox10. Scale bar, 25 μm. (g) Percentage of cl-Casp3⁺/Sox10⁺ cells from above wild-type OPCs treated with FasL. Data represent the mean±s.e.m. from three independent experiments. ***P<0.001; Student's t-test.