Figure 1. A stretch-activated TRPC5-like channel in rat baroreceptor neurons.

Representative traces (a-d), their corresponding event histograms (b-d) and summarized single-channel open probabilities (NP_o, e-f) showing a stretch-activated channel. The recordings were made in excised inside-out patches (i/o) from the neurite terminals of DiI-labelled primary cultured aortic baroreceptor neurons with a holding potential at −60 mV. (a) Continuous recording of single-channel current trace before and after negative pressure of −30 mm Hg. (b,e) Representative traces from a single patch under different pressures (b) and data summary (e). (c,d,f) Effect of T5DN (c and f, vector as control) and T5E3 (d and f, preimmune IgG as control) on the channel activities at −30 mm Hg pressure. NP_o were summarized from multiple patches. (g) Single-channel I−V relationship of the stretch-activated channel. Mean±s.e.m. ^&P<0.05 as compared with 0 mm Hg, *P<0.05 as compared with preimmune IgG, ^#P<0.05 as compared with vector, statistical analysis was performed by Student’s t-test.