Figure 2. TRPC5 channel in the hypertonicity-induced whole-cell current.

(a,b) Whole-cell I–V relationships of a representative single primary cultured aortic baroreceptor neurons in isotonic (300 mOsm) and hypotonic (240 mOsm) conditions in the absence (a) and presence (b) of 20 μmol l⁻¹ GdCl₃. I–V relationship was obtained using a ramp protocol from −100 mV to +100 mV with 500-ms duration and repeated every 1 s. (c) Summary of data showing the hypoosmolarity-activated whole-cell current density at ±80 mV under different conditions. Subtraction of currents in isotonic condition from the hypotonic one yielded the hypoosmolarity-activated currents (Δ current density). T5E3, 15 μg ml⁻¹; T5DN, T5DN transfection; 2-APB, 100 μmol l⁻¹. Mean±s.e.m. ^‡P<0.01 as compared with no-treatment, ^&&P<0.01 as compared with GdCl₃, ^**P<0.01 as compared with preimmune IgG and ^#P<0.05 as compared with vector, statistical analysis was performed by Student’s t-test.