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. 2016 Jul 14;7:11947. doi: 10.1038/ncomms11947

Figure 3. Endogenous TRPC5 expression in rat aortic baroreceptor neurons.

Figure 3

(a) A schematic diagram showing the positions of left nodose ganglion, aortic depressor nerve and baroreceptor terminals in aortic arch. Tissue sections and aortic arch adventitia were obtained at the positions as indicated by dashes and scissors. (b) Representative images of immunoblots (n=3) showing endogenous expression of TRPC5 proteins in left nodose ganglion (left lane). Lysate from TRPC5-overexpressing HEK293 cells were used as positive control (right lane). (c) RT–PCR detection of Trpc5 mRNA in left nodose ganglion. Shown were the RT–PCR products of Trpc5 and the DNA ladder (n=3). (d–f) Representative images (n=3) showing the immunoreactivity to TRPC5 (green), a neuronal marker PGP9.5 (red, d–f) at the left nodose ganglion (d), aortic depressor nerve (e) and baroreceptor terminals within the aortic arch adventitia (f). Also shown were the merged images of TRPC5 and PGP9.5. (g) Representative images (n=3) showing the immunoreactivity to TRPC5 (green) and a myelin-marker myelin basic protein (MBP) at aortic depressor nerve. Controls were without primary antibodies. In the merged image, arrowheads show the TRPC5 expression in myelinated fibre, whereas asterisks illustrate the TRPC5 expression in unmyelinated fibres. (h) T5E3 labelling (green) of DiI-positive (red) aortic baroreceptor neurons (n=4). These were non-permeabilized neurons in primary culture.