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. 2016 Jul 15;7:12226. doi: 10.1038/ncomms12226

Figure 3. Deletion of the Arp3 C-terminal tail causes defects in endocytic actin patches and in the uptake of FM4-64.

Figure 3

(a) Spinning disk confocal fluorescence micrographs of wild type (WT) and mutant S. pombe cells expressing Fim1-GFP. Images are taken thorough the middle of the cells. Scale bar, 2 μm. (b) Average number of patches per cell (n≥50 cells). Error bars show s.e. (c) Kymographs of four actin patches for wild type and arp3ΔC strain. (d) Average patch lifetime for wild type and arp3ΔC strains. Error bars show s.e. (e) Quantification of Fim1-GFP intensity in WT and arp3ΔC S. pombe cells. Data show average intensity and s.d. from measurements of patches from wt (n=30 patches) and arp3ΔC (n=30) strains, respectively. The zero time point was taken to be the frame of maximum Fim1-GFP intensity during the lifetime of each patch. (f) FM4-64 endocytosis assay showing that arp3ΔC strain exhibits slowed dye uptake compared with the wild-type strain. Scale bar, 2 μm.