Figure 2. Regulation of actomyosin contractility and pacsin2 recruitment to FAJs.

Widefield IF images of HUVECs treated with thrombin (0.2 U ml⁻¹, 10 min) or the Rho-kinase inhibitor Y-27632 (10 μM, 10 min) as described previously²⁴. Staining for pacsin2 is in green, F-actin in red and VE-cadherin in blue. The right panel represents enlargements of regions of interests from the merged images. Graph shows the average percentage±s.e.m. of FAJs that are pacsin2 positive from n≥2 independent experiments (control untreated HUVECs n=22 images; thrombin-treated HUVECs n=21 images; and Y-27632-treated HUVECs n=17 images). **P≤0.01 (Student's t-test).