Fig 3.

CEA inhibits autophagy induced by 5-FU. A) SW742- and B) CHO-CEA transfectants as well as control parental cells were treated with 5-FU for 72 h. After staining with acridine orange, cells were examined by fluorescence microscopy. The right figure in both panels is 5-FU-untreated parental cells. Acridine positive, bright fluorescent-stained vacuoles showed significant increase in autophagic cells in 5-FU treated parental cell populations, but very few in CEA-transfected cells. Photomicrographs are the representatives of three independent experiments.